PreparationZombie Violet™ Fixable Viability Kit is composed of lyophilized Zombie Violet™ dye and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 100 µl of DMSO to one vial of Zombie Violet™ dye until fully dissolved. 100 tests = 1 vial of Zombie Violet™ + DMSO, 500 tests = 5 vials of Zombie Violet™ + DMSO.

Storage & HandlingStore kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Zombie Violet™ dye, use immediately, or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than one month.

Application

FC, ICFC - Quality tested

ICC - Verified

Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometry, the suggested dilution is 1:100-1:1000 for 1-10 million cells. For immunocytochemistry, the suggested dilution is 1:1000. It is recommended that the reagent be titrated for optimal performance for each application, as optimal dosage varies with cell type.

Excitation LaserViolet Laser (405 nm)

Application Notes

Zombie Violet™ dye is excited by the Violet laser (405 nm) and has fluorescence emission maximum at 423 nm. If using in a multi-color panel design, filter optimization may be required depending on other fluorophores used. Zombie Violet™ dye has similar emission to BV421.