WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
| 1:1,000-1:2,000 |
- |
1:50-1:200 |
1:50-1:200 |
1:50-1:200 |
- |
- |
- |
抗体详细信息如下:
| About this Antibody |
| Name |
CaMKII Rabbit Monoclonal Antibody |
| Category |
Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
| Isotype |
IgG |
| Purification |
Affinity purification |
| About the Immunogen |
| Immunogen |
Recombinant protein |
| Gene ID |
816/818/817/815(Human); 12322/12323/108058/12325(Mouse); 24245/25400/171140/24246(Rat) |
| SwissProt |
Q13554/Q13555/Q13557/Q9UQM7(Human); P11798/P28652/Q6PHZ2/Q923T9(Mouse); P08413/P11275/P11730/P15791(Rat) |
| Synonyms |
CAMK2B; CAM2; CAMK2; CAMKB; CAMK2G; CAMK2D; CAMK2A |
| Category |
Neuronal Signaling |
| Background |
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
包装清单:
| 产品编号 |
产品名称 |
包装 |
| AF1639 |
CaMKII Rabbit Monoclonal Antibody |
50μl |
| AZ050 |
Western一抗稀释液 |
50ml |
| - |
说明书 |
1份 |
保存条件:
CaMKII Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
