Antimicrobial agent for primary cells

Primocin® is designed to offer complete protection of primary cells from microbial contamination. Primocin® is a broad-spectrum antibiotic formulation that is gentle on your cells but lethal to the microbes. There are a number of common sources of microbial contamination especially in the isolation of primary cells from both animal and human tissue, including commensal flora and/or subclinical infections.

Use of Primocin® in primary cell cultures

Key Features:

• Active against bacteria, mycoplasma, and fungi
• A common addition to various standardized primary cell culture protocols
• Used throughout culturing process (e.g. culture media, wash solutions, and  biopsy storage media) 
• Highly cited in the literature

Primocin® is composed of four compounds, of which three, act directly on Gram-positive bacteria, Gram-negative bacteria, and mycoplasmas. The primary mode of action for these compounds is to block microbial DNA and protein synthesis. The fourth compound in Primocin® eradicates fungi, including yeasts, by directly targeting processes at the fungal membrane.

Primocin® is successfully used in many types of primary cell cultures (see citations) including:

• Differentiated mouse- and human-derived cells such as astrocytes and immune cells (e.g. natural killer cells)
• Stem cells such as mouse- and human-derived embryonic and pluripotent stem cells
• 3D cellular models (i.e. organoid and spheroids) such as colon epithelial and carcinoma organoids

Primocin® is made in-house at InvivoGen and is of the highest quality with rigorous quality control performed to ensure the absence of lot-to-lot variation.

Note: Primocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents.

All InvivoGen products are for internal research use only, and not for human or veterinary use.